2 articles published in JoVE
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation Qin Fu1, Casey W. Johnson1, Bhagya K. Wijayawardena2, Michael P. Kowalski2, Miranda Kheradmand2, Jennifer E. Van Eyk1 1Advanced Clinical Biosystems Institute, Smidt Heart institute, Cedars Sinai Medical Center, 2Beckman Coulter Life Sciences Here, we present an automated plasma protein digestion method for mass spectrometry-based quantitative proteomic analysis. In this protocol, the liquid transferring and incubation steps for protein denaturation, reduction, alkylation, and trypsin digestion reactions are streamlined and automated. It takes approximately five hours to prepare a 96-well plate with desired precision.
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood Yoshinobu Koguchi1, Iliana L. Gonzalez1, Tanisha L. Meeuwsen1, William L. Miller1, Daniel P. Haley1,2, Alice N. Tanibata-Branham3, Keith S. Bahjat1,4 1Human Immune Monitoring Laboratory, Earle A. Chiles Research Institute, Providence Cancer Center, Providence Portland Medical Center, 2Sony Biotechnology, 3Beckman Coulter, Inc. Life Sciences, 4Bristol-Myers Squibb Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.