2 articles published in JoVE
Microinjection of DNA into Eyebuds in Xenopus laevis Embryos and Imaging of GFP Expressing Optic Axonal Arbors in Intact, Living Xenopus Tadpoles Sophia Dao1, Kenton Jones1, Tamira Elul1 1College of Osteopathic Medicine, Touro University California This protocol aims to demonstrate how to microinject a DNA/DOTAP mixture into eyebuds of one day old Xenopus laevis embryos, and how to image and reconstruct individual green fluorescent protein (GFP) expressing optic axonal arbors in tectal midbrains of intact, living Xenopus tadpoles.
Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate Shona A. Mookerjee1,2, Martin D. Brand1,2 1Touro University California College of Pharmacy, 2Buck Institute for Research on Aging Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.