3 articles published in JoVE
Multimodal Bioluminescent and Positronic-emission Tomography/Computational Tomography Imaging of Multiple Myeloma Bone Marrow Xenografts in NOG Mice Gilbert Gastelum1, Eric Y. Chang2, David Shackleford3, Nicholas Bernthal3, Jeffery Kraut1,3, Kevin Francis4, Victoria Smutko1, Patrick Frost1,3 1Greater Los Angeles Veteran Administration Healthcare System, 2San Diego Veterans Administration Healthcare System, 3University of California, Los Angeles, 4Perkin Elmer Here we use bioluminescent, X-ray, and positron-emission tomography/computed tomography imaging to study how inhibiting mTOR activity impacts bone marrow-engrafted myeloma tumors in a xenograft model. This allows for physiologically relevant, non-invasive, and multimodal analyses of the anti-myeloma effect of therapies targeting bone marrow-engrafted myeloma tumors in vivo.
A Radio-telemetric System to Monitor Cardiovascular Function in Rats with Spinal Cord Transection and Embryonic Neural Stem Cell Grafts Shaoping Hou1, Armin Blesch2, Paul Lu3,4 1Spinal Cord Research Center, Department of Neurobiology & Anatomy, Drexel University College of Medicine, 2Spinal Cord Injury Center, Heidelberg University Hospital, 3Veterans Administration Medical Center, San Diego, CA, 4Department of Neurosciences, University of California, San Diego We present a protocol for using a radio-telemetric system to record cardiovascular parameters in T4 spinal cord transected rats eight weeks after embryonic brainstem neural stem cell grafting into the lesion site. Telemetry is an advanced technique to accurately evaluate cardiovascular function in conscious freely moving spinal cord injured rats.
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons Xiaobei Zhao1, Yue Zhou2, April M. Weissmiller1, Matthew L. Pearn3,4, William C. Mobley1, Chengbiao Wu1 1Department of Neurosciences, University of California, San Diego, 2School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, 3Department of Anesthesiology, University of California, San Diego, 4VA San Diego Healthcare System Axonal transport of BDNF, a neurotrophic factor, is critical for the survival and function of several neuronal populations. Some degenerative disorders are marked by disruption of axonal structure and function. We demonstrated the techniques used to examine live trafficking of QD-BDNF in microfluidic chambers using primary neurons.