Department of Molecular Medicine 1 article published in JoVE Developmental Biology Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants Megan Vaughan1, Katja A. Lamia1 1Scripps Research, Department of Molecular Medicine Myoblasts are proliferating precursor cells that differentiate to form polynucleated myotubes and eventually skeletal muscle myofibers. Here, we present a protocol for efficient isolation and culture of primary myoblasts from young adult mouse skeletal muscles. The method enables molecular, genetic, and metabolic studies of muscle cells in culture.
