United States Department of Agriculture, Albany, CA 1 article published in JoVE Bioengineering High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay Jana A. Hassan1, Ilea J. Chau-Ly1, Jennifer D. Lewis1,2 1Department of Plant and Microbial Biology, University of California, Berkeley, 2Plant Gene Expression Center, United States Department of Agriculture The seedling flood assay facilitates rapid screening of wild tomato accessions for the resistance to the Pseudomonas syringae bacterium. This assay, used in conjunction with the seedling bacterial growth assay, can assist in further characterizing the underlying resistance to the bacterium, and can be used to screen mapping populations to determine the genetic basis of resistance.
