2 articles published in JoVE
Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins Scot R. Weinberger1, Emily E. Chea1, Joshua S. Sharp1,2,3, Sandeep K. Misra2 1GenNext Technologies Inc., 2Department of Biomolecular Sciences, School of Pharmacy, University of Mississippi, 3Department of Chemistry and Biochemistry, University of Mississippi This protocol presents a method to use inline radical dosimetry and a plasma light source to perform flash oxidation protein footprinting. This method replaces the hazardous UV laser to simplify and improve the reproducibility of fast photochemical oxidation of protein studies.
Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes Sandeep K. Misra1, Joshua S. Sharp1,2,3 1Department of Biomolecular Sciences, University of Mississippi, 2Department of Chemistry and Biochemistry, University of Mississippi, 3GenNext Technologies, Inc. Fast photochemical oxidation of proteins is an emerging technique for the structural characterization of proteins. Different solvent additives and ligands have varied hydroxyl radical scavenging properties. To compare the protein structure in different conditions, real-time compensation of hydroxyl radicals generated in the reaction is required to normalize reaction conditions.