Molecular Biology Institute at UCLA 1 article published in JoVE Biology Determining Basal Energy Expenditure and the Capacity of Thermogenic Adipocytes to Expend Energy in Obese Mice Michael Shum1, Zhiqiang Zhou2, Marc Liesa2,3,4 1Department of Molecular Medicine, Faculty of Medicine, Universite Laval, 2Department of Medicine, Division of Endocrinology, David Geffen School of Medicine at UCLA, 3Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 4Molecular Biology Institute at UCLA This manuscript describes a protocol to measure the basal metabolic rate and the oxidative capacity of thermogenic adipocytes in obese mice.