2 articles published in JoVE
Fluorescent Orthotopic Mouse Model of Pancreatic Cancer Jonathan A. Moreno1, Antonio Sanchez2, Robert M. Hoffman3,4, Saima Nur1, Maria P. Lambros1 1College of Pharmacy, Western University of Health Sciences, 2UVP, LLC, 3Department of Surgery, School of Medicine, University of California San Diego, 4Anticancer Inc. A procedure to implant green fluorescent protein-expressing pancreatic cancer cells (PANC-1 GFP) orthotopically into the pancreas of Balb-c Ola Hsd-Fox1nu mice to assess tumor progression and metastasis is presented here.
Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction Robert M. Hoffman1, Lingna Li1 1AntiCancer, Inc. The hair follicle is a highly complex appendage of the skin containing a multiplicity of cell types. The follicle undergoes constant cycling through the life of the organism including growth and resorption with growth dependent on specific stem cells. The targeting of the follicle by genes and stem cells to change its properties, in particular, the nature of the hair shaft is, discussed. Hair follicle delivery systems are described, such as liposomes and viral vectors for gene therapy. The nature of the hair follicle stem cells is discussed, in particular, its pluripotency.