3 articles published in JoVE
Fluorescent Orthotopic Mouse Model of Pancreatic Cancer Jonathan A. Moreno1, Antonio Sanchez2, Robert M. Hoffman3,4, Saima Nur1, Maria P. Lambros1 1College of Pharmacy, Western University of Health Sciences, 2UVP, LLC, 3Department of Surgery, School of Medicine, University of California San Diego, 4Anticancer Inc. A procedure to implant green fluorescent protein-expressing pancreatic cancer cells (PANC-1 GFP) orthotopically into the pancreas of Balb-c Ola Hsd-Fox1nu mice to assess tumor progression and metastasis is presented here.
Staining Proteins in Gels Sean Gallagher1, Deb Chakavarti2 1Keck Graduate Institute of Applied Life Sciences, UVP, LLC, 2Proteomic Center, Keck Graduate Institute of Applied Life Sciences Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. Staining of proteins with Coomassie Blue, Silver Staining, SYPRO Orange, SYPRO Ruby are demonstrated in this video.
Immunoblot Analysis Sean Gallagher1, Deb Chakavarti2 1UVP, LLC, 2Proteomic Center, Keck Graduate Institute of Applied Life Sciences Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. This video provides protocols for protein separation, blotting proteins onto membranes, immunoprobing, and visualization using chromogenic or chemiluminescent substrates.