Keck Graduate Institute of Applied Life SciencesView Institution's Website
3 articles published in JoVE
Staining Proteins in Gels Sean Gallagher1, Deb Chakavarti2 1Keck Graduate Institute of Applied Life Sciences, UVP, LLC, 2Proteomic Center, Keck Graduate Institute of Applied Life Sciences Following separation by electrophoretic methods, proteins in a gel can be detected by several staining methods. Staining of proteins with Coomassie Blue, Silver Staining, SYPRO Orange, SYPRO Ruby are demonstrated in this video.
Immunoblot Analysis Sean Gallagher1, Deb Chakavarti2 1UVP, LLC, 2Proteomic Center, Keck Graduate Institute of Applied Life Sciences Immunoblotting (western blotting) is a rapid and sensitive assay for the detection and characterization of proteins that works by exploiting the specificity inherent in antigen-antibody recognition. This video provides protocols for protein separation, blotting proteins onto membranes, immunoprobing, and visualization using chromogenic or chemiluminescent substrates.