2 articles published in JoVE
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function Mia J. Smith1,2, Thomas A. Packard1, Shannon K. O'Neill1, Rochelle M. Hinman1, Marynette Rihanek3, Peter A. Gottlieb3, John C. Cambier1,4 1Department of Immunology and Microbiology, University of Colorado School of Medicine, 2Department of Microbiology, Immunology, and Pathology, Colorado State University, 3Barbara Davis Center for Childhood Diabetes, University of Colorado School of Medicine, 4Department of Biomedical Research, National Jewish Health A simple yet effective method that employs magnetic nanoparticles to detect and enrich antigen-reactive B cells for functional and phenotypic analysis is described.
Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry Manira Rayamajhi1, Elizabeth F. Redente2, Tracy V. Condon1, Mercedes Gonzalez-Juarrero3, David W.H. Riches1,2,4, Laurel L. Lenz1,4 1Department of Immunology, University of Colorado School of Medicine, 2Division of Cell Biology, Department of Pediatrics, National Jewish Health, 3Department of Microbiology, Immunology, and Pathology, Colorado State University, 4Department of Immunology, National Jewish Health We illustrate non-surgical delivery of test materials into the lungs of anesthetized mice via the trachea. This method permits lung exposure to bacterial and viral pathogens, cytokines, antibodies, beads, chemicals, or dyes. We further describe harvesting and processing of lungs and lung draining lymph nodes (LDLNs) for flow cytometry.