Children's Hospital Colorado 2 articles published in JoVE Neuroscience Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation Stacey M. Thomas1, Joseph Bednarek2, William J. Janssen1, Patrick S. Hume1 1Division of Pulmonary, Sleep and Critical Care Medicine, Department of Medicine, National Jewish Health, 2 Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies. Immunology and Infection Transurethral Instillation Procedure in Adult Male Mouse Sanghee Lee*1, Alonso Carrasco Jr.*2, Randall B. Meacham1, Anna P. Malykhina1 1Division of Urology, Department of Surgery, University of Colorado School of Medicine, 2 Transurethral instillation is a challenging procedure and has not been well described in the literature. The aim of this manuscript is to describe a technique for transurethral insertion of a catheter for intravesical delivery of liquids with active substances into the urinary bladder and/or prostate in adult male mice.
