2 articles published in JoVE
Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining Wells B. LaRiviere1,2, Xiaorui Han3,4, Kaori Oshima1, Sarah A. McMurtry1, Robert J. Linhardt3, Eric P. Schmidt1,5 1Department of Medicine, University of Colorado Anschutz Medical Campus, 2Medical Scientist Training Program, University of Colorado Anschutz Medical Campus, 3Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 4Department of Health Sciences, Curtin University, 5Department of Medicine, Denver Health Medical Center This report describes techniques to isolate and purify sulfated glycosaminoglycans (GAGs) from biological samples and a polyacrylamide gel electrophoresis approach to approximate their size. GAGs contribute to tissue structure and influence signaling processes via electrostatic interaction with proteins. GAG polymer length contributes to their binding affinity for cognate ligands.
Direct Intrabronchial Administration to Improve the Selective Agent Deposition Within the Mouse Lung Sumei Liao1, Oliver Eickelberg1, Eric P. Schmidt1,2, Yimu Yang1 1Department of Medicine, University of Colorado Denver, 2Department of Medicine, Denver Health Medical Center Intratracheal (IT) administration of experimental agents in mice often results in asymmetric delivery to the distal lungs. In this report, we describe a direct intrabronchial (IB) approach to cannulate each lung in living mice non-operatively. This approach can be used to selectively administer agents to one lung or may be adapted to improve the symmetric agent delivery to both lungs.