2 articles published in JoVE
Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay Philip M. Armstrong1, Theodore G. Andreadis1, Shannon L. Finan1, John J. Shepard1, Michael C. Thomas1 1Center for Vector Biology and Zoonotic Diseases, Department of Environmental Sciences, The Connecticut Agricultural Experiment Station We describe a method to process and screen field-collected mosquitoes for a diversity of viruses by Vero cell culture assay. By employing this technique, we have detected 9 different viruses from 4 taxonomic families in mosquitoes collected in Connecticut.
Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut Toru Kariu1, Adam S. Coleman1, John F. Anderson2, Utpal Pal1 1Department of Veterinary Medicine, University of Maryland, 2Department of Entomology, Connecticut Agricultural Experiment Station Lyme disease research studies often require generation of ticks infected with the pathogen Borrelia burgdorferi, a process that typically takes several weeks. Here we demonstrate a microinjection-based tick infection procedure that can be accomplished within hours. We also demonstrate an immunofluorescence method for in situ localization of B. burgdorferi within ticks.