2 articles published in JoVE
Ultra-long Read Sequencing for Whole Genomic DNA Analysis Liang Gong1, Chee-Hong Wong1, Jennifer Idol1, Chew Yee Ngan1, Chia-Lin Wei1 1Genome Technologies, Jackson Laboratory for Genomic Medicine Long-read sequences greatly facilitate the assembly of complex genomes and characterization of structural variation. We describe a method to generate ultra-long sequences by nanopore-based sequencing platforms. The approach adopts an optimized DNA extraction followed by modified library preparations to generate hundreds of kilobase reads with moderate coverage from human cells.
An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues Christopher Terranova1, Ming Tang1, Elias Orouji1, Mayinuer Maitituoheti1, Ayush Raman1, Samirkumar Amin2, Zhiyi Liu1, Kunal Rai1 1Department of Genomic Medicine, University of Texas MD Anderson Cancer Center, 2The Jackson Laboratory for Genomic Medicine Here, we describe an optimized high-throughput ChIP-sequencing protocol and computational analyses pipeline for the determination of genome-wide chromatin state patterns from frozen tumor tissues and cell lines.