2 articles published in JoVE
An Explant Assay for Assessing Cellular Behavior of the Cranial Mesenchyme Anjali A. Sarkar1, Irene E. Zohn1 1Center for Neuroscience Research, Children's Research Institute, Children's National Medical Center The cranial mesenchyme undergoes dramatic morphogenic movements that likely provides a driving force for elevation of the neural folds1,2. Here we describe a simple ex vivo explant assay to characterize the cellular behaviors of the cranial mesenchyme during neurulation. This assay has numerous applications including being amenable to pharmacological manipulations and live imaging analyses.
Labeling and Imaging Cells in the Zebrafish Hindbrain Pradeepa Jayachandran1, Elim Hong2, Rachel Brewster1 1Department of Biological Sciences, University of Maryland, Baltimore County, 2Center for Neuroscience, Children's National Medical Center Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.