3 articles published in JoVE
Novel Sequence Discovery by Subtractive Genomics Kathryn C. Asalone1, Megan M. Nelson1, John R. Bracht1 1Biology Department, American University The purpose of this protocol is to use a combination of computational and bench research to find novel sequences that cannot be easily separated from a co-purifying sequence, which may be only partially known.
Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis Katie L. DeCicco-Skinner1, Gervaise H. Henry1, Christophe Cataisson2, Tracy Tabib1, J. Curtis Gwilliam1, Nicholas J. Watson1, Erica M. Bullwinkle1, Lauren Falkenburg1, Rebecca C. O'Neill1, Adam Morin1, Jonathan S. Wiest2 1Department of Biology, American University, 2Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH The tube formation assay is a fast, quantifiable method for measuring in vitro angiogenesis. Endothelial cells are combined with conditioned media and plated on basement membrane extract. Tube formation occurs within hours and newly formed tubules easily quantified.
Isolation and Primary Culture of Rat Hepatic Cells Ling Shen1, Allix Hillebrand2, David Q.-H. Wang3, Min Liu1 1Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, 2American University in Washington, D.C., 3Department of Internal Medicine, Saint Louis University School of Medicine Primary hepatocytes provide a valuable tool to evaluate biochemical, molecular, and metabolic functions in a physiologically relevant experimental system. We describe a reliable protocol for rat in situ liver perfusion, which consistently generates viable hepatocytes up to 1.0 × 108 cells per preparation with cell viability between 88 ~ 96%.