3 articles published in JoVE
A Mouse Model of Single and Repetitive Mild Traumatic Brain Injury Bevan S. Main1, Stephanie S. Sloley1, Sonia Villapol1, David N. Zapple2, Mark P. Burns1 1Laboratory for Brain Injury and Dementia, Department of Neuroscience, Georgetown University Medical Center, 2University Information Systems, Division of Research Technologies, Georgetown University Athletes absorb several hundred mild traumatic brain injuries (mTBI)/concussions every year; however, the consequence of these on the brain is poorly understood. Therefore, an animal model of single and repetitive mTBI that consistently replicates clinically relevant symptoms provides the means to advance the study of mTBI and concussion.
Live Cell Imaging and 3D Analysis of Angiotensin Receptor Type 1a Trafficking in Transfected Human Embryonic Kidney Cells Using Confocal Microscopy Parnika Kadam1,2, Ryan McAllister3, Jeffrey S. Urbach3, Kathryn Sandberg1,2, Susette C. Mueller4 1Department of Biochemistry, Georgetown University Medical Center, 2Department of Medicine, Georgetown University Medical Center, 3Department of Physics, Georgetown University Medical Center, 4Department of Oncology, Georgetown University Medical Center Here we present a protocol to image cells expressing green fluorescent protein-tagged angiotensin type 1a receptors during endocytosis initiated by angiotensin II treatment. This technique includes labeling lysosomes with a second fluorescent marker, and then utilizing software to analyze the co-localization of receptor and lysosome in three dimensions over time.
A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation Lucas Tricoli1, Deborah L. Berry2, Chris Albanese1 1Department of Oncology, Lombardi Comprehensive Cancer Center, 2Department of Oncology, Georgetown University Medical Center Here, we present a method for the establishment of a rapid in vitro system that supports the three dimensional culturing and subsequent luminal differentiation of primary prostate epithelial cells.