American Red Cross, National Headquarters 1 article published in JoVE Biology Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts Meiling Melzer1, David Beier1, Pampee P. Young1,2, Sarika Saraswati1 1Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, 2American Red Cross, National Headquarters Obtaining a pure population of fibroblasts is crucial to studying their role in wound repair and fibrosis. Described here is a detailed method to isolate fibroblasts and myofibroblasts from uninjured and injured mouse hearts followed by characterization of their purity and functionality by immunofluorescence, RTPCR, fluorescence-assisted cell sorting, and collagen gel contraction.
