3 articles published in JoVE
In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages Alyssa J Rolfe1, Dale B Bosco1, Erynn N Broussard1, Yi Ren1 1Department of Biomedical Sciences, College of Medicine, Florida State University We present methods to assess the phagocytic capacity of primary murine bone marrow-derived macrophages using fluorescently labeled myelin debris and intracellular lipid droplet staining.
Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition Jeremy J. Flint1,2, Kannan Menon2,3, Brian Hansen4, John Forder2,3,5, Stephen J. Blackband1,2,6 1Department of Neuroscience, University of Florida, 2McKnight Brain Institute, University of Florida, 3Department of Biomedical Engineering, University of Florida, 4Center for Functionally Integrative Neuroscience, Aarhus University, 5Department of Radiology, University of Florida, 6National High Magnetic Field Laboratory, Florida State University The current protocol describes a method by which users can maintain viability of acute hippocampal and cortical slice preparations during the collection of magnetic resonance microscopy data.
Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro Rafaela G. Feresin1, Shirin Pourafshar2,3, Jingwen Huang2, Yitong Zhao2, Bahram H. Arjmandi2,3, Gloria Salazar2,3 1Department of Dietetics and Nutrition, University of Arkansas for Medical Sciences, 2Department of Nutrition, Food and Exercise Sciences, Florida State University, 3Center for Advancing Exercise and Nutrition Research on Aging (CAENRA), Florida State University This work details a step-by-step method to prepare polyphenol-rich extracts from freeze-dried berry powder. In addition, it provides a thorough description of how to use these polyphenol-rich extracts in cell culture in the presence of the peptide hormone angiotensin II (Ang II) using Vascular Smooth Muscle Cells (VSMCs).