NeoGenomics Laboratories 1 article published in JoVE Genetics Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations Adam Z. Albitar1, Wanlong Ma1, Maher Albitar1 1NeoGenomics Laboratories Wild-type blocking PCR followed by direct sequencing offers a highly sensitive method of detection for low frequency somatic mutations in a variety of sample types.