3 articles published in JoVE
Visualizing Efficacy of Pesticides Against Disease Vector Mosquitoes in the Field Seth C. Britch1, Kenneth J. Linthicum1, Robert L. Aldridge1, Frances V. Golden1, Todd W. Walker2 1Mosquito & Fly Research Unit, United States Department of Agriculture, Agricultural Research Service Center for Medical, Agricultural, & Veterinary Entomology, 2East Baton Rouge Mosquito Abatement and Rodent Control The efficacy of public health pesticides targeting nuisance and disease-vector insects is not uniform across different ecological zones. Here we present a system of techniques using captive vector insects as sentinels for pesticide efficacy to derive electronic maps supporting the standard evaluation of pesticides across multiple environments.
Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies Neil D. Sanscrainte1, Christy M. Waits2,3, Christopher J. Geden1, Alden S. Estep1,2, James J. Becnel1 1USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, 2CMAVE Detachment, Navy Entomology Center of Excellence, 3Lovelace Respiratory Research Institute This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.
Key Elements of Photo Attraction Bioassay for Insect Studies or Monitoring Programs Lee W. Cohnstaedt1, Jordan C. Disberger1, Eric Paulsen2, Adrian J. Duehl3 1Arthropod-Borne Animal Diseases Research Unit, Center for Grain and Animal Health Research, Agricultural Research Service, United States Department of Agriculture, 2Center for Medical, Agricultural, and Veterinary Entomology, Agricultural Research Service, United States Department of Agriculture, 3Bayer Photo-attraction bioassay arenas are used to determine the optimal light color(s) to maximize insect attraction; however bioassays and methods are specific to target insect behaviors and habitats. Customizable equipment and modifications are explained for nocturnal or diurnal and terrestrial or aerial insects.