2 articles published in JoVE
Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice Alessandro F. Ulivi1, Tim P. Castello-Waldow1, Ghabiba Weston1,2, Long Yan3, Ryohei Yasuda3, Alon Chen1,4, Alessio Attardo1 1Dept. of Stress Neurobiology and Neurogenetics, Max Planck Institute of Psychiatry, 2Graduate School of Systemic Neurosciences, Ludwig Maximilians University, 3Max Planck Florida Institute for Neuroscience, 4Dept. of Neurobiology, Weizmann Institute of Science This method describes a chronic preparation that allows optical access to the hippocampus of living mice. This preparation can be used to perform longitudinal optical imaging of neuronal structural plasticity and activity-evoked cellular plasticity over a period of several weeks.
Ex Vivo Optogenetic Dissection of Fear Circuits in Brain Slices Daniel Bosch1, Douglas Asede2, Ingrid Ehrlich1 1Hertie Institute for Clinical Brain Research and Werner Reichardt Centre for Integrative Neuroscience, University of Tuebingen, 2Max Planck Florida Institute for Neuroscience Optogenetic approaches are widely used to manipulate neural activity and assess the consequences for brain function. Here, a technique is outlined that upon in vivo expression of the optical activator Channelrhodopsin, allows for ex vivo analysis of synaptic properties of specific long range and local neural connections in fear-related circuits.