3 articles published in JoVE
Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone James Weger-Lucarelli1, Nisha K. Duggal2, Aaron C. Brault2, Brian J. Geiss1, Gregory D. Ebel1 1Department of Microbiology, Immunology, and Pathology, Colorado State University, 2Division of Vector-Borne Diseases, Centers for Disease Control and Prevention This protocol describes the recovery of infectious Zika virus from a two-plasmid infectious cDNA clone.
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro Jennifer L. Harcourt1, Lia M. Haynes1 1National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC) The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.
Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks Toni G. Patton1, Gabrielle Dietrich2, Kevin Brandt1, Marc C. Dolan2, Joseph Piesman2, Robert D. Gilmore Jr.1 1Microbiology and Pathogenesis Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, 2Tick-Borne Diseases Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention The collection of infected tick hemolymph, salivary glands, and saliva is important to study how tick-borne pathogens cause disease. In this protocol we demonstrate how to collect hemolymph and salivary glands from feeding Ixodes scapularis nymphs. We also demonstrate saliva collection from female I. scapularis adults.