4 articles published in JoVE

• Immunology and Infection

  
  Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells F. Liaini Gross^1,2, Yaohui Bai¹, Stacie Jefferson¹, Crystal Holiday¹, Min Z. Levine^{1 1}Influenza Division, Centers for Disease Control and Prevention, ²Battelle Influenza neutralizing antibodies correlate with protection of influenza infections. Microneutralization assays measure neutralizing antibodies in human sera and are often used for influenza human serology. We describe a microneutralization assay using MDCK-SIAT1 cells to measure neutralizing antibody titers to contemporary 3C.2a and 3C.3a A(H3N2) viruses following influenza vaccination or infection.

• Immunology and Infection

  
  Rescue and Characterization of Recombinant Virus from a New World Zika Virus Infectious Clone James Weger-Lucarelli¹, Nisha K. Duggal², Aaron C. Brault², Brian J. Geiss¹, Gregory D. Ebel^{1 1}Department of Microbiology, Immunology, and Pathology, Colorado State University, ²Division of Vector-Borne Diseases, Centers for Disease Control and Prevention This protocol describes the recovery of infectious Zika virus from a two-plasmid infectious cDNA clone.

• Immunology and Infection

  
  Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro Jennifer L. Harcourt¹, Lia M. Haynes^{1 1}National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC) The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

• Immunology and Infection

  
  Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks Toni G. Patton¹, Gabrielle Dietrich², Kevin Brandt¹, Marc C. Dolan², Joseph Piesman², Robert D. Gilmore Jr.^{1 1}Microbiology and Pathogenesis Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, ²Tick-Borne Diseases Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention The collection of infected tick hemolymph, salivary glands, and saliva is important to study how tick-borne pathogens cause disease. In this protocol we demonstrate how to collect hemolymph and salivary glands from feeding Ixodes scapularis nymphs. We also demonstrate saliva collection from female I. scapularis adults.