3 articles published in JoVE
Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes Andrew S. Riching1, Yuanbiao Zhao1, Yingqiong Cao1, Pilar Londono1, Hongyan Xu2, Kunhua Song1 1Division of Cardiology, Department of Medicine, University of Colorado Anschutz Medical Campus, 2Department of Population Health Sciences, Medical College of Georgia, Augusta University Here we present a robust method to reprogram primary embryonic fibroblasts into functional cardiomyocytes through overexpression of GATA4, Hand2, Mef2c, Tbx5, miR-1, and miR-133 (GHMT2m) alongside inhibition of TGF-β signaling. Our protocol generates beating cardiomyocytes as early as 7 days post-transduction with up to 60% efficiency.
An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis Amy V. Paschall1,2,3, Kebin Liu1,2,3 1Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, 2Georgia Cancer Center, Augusta University, 3Charlie Norwood VA Medical Center An orthotopic breast cancer primary tumor model and surgical removal of primary tumor to extend mouse life to generate spontaneous metastasis are described. The tumor growth and progression are monitored and quantified by luciferase fluorescence imaging.
Ultrasound Assessment of Endothelial Function: A Technical Guideline of the Flow-mediated Dilation Test Paula Rodriguez-Miguelez1, Nichole Seigler1, Ryan A. Harris1,2 1Division of Clinical and Translational Sciences, Georgia Prevention Institute, Georgia Regents University, 2Sport and Exercise Science Research Institute, University of Ulster The flow mediated dilation (FMD) test is the most commonly utilized, non-invasive, ultrasound assessment of endothelial function in humans. Although the FMD test has been related with the prediction of future cardiovascular disease and events, it is a physiological assessment with many inherent confounding factors that need to be considered.