Parker H. Petit Institute for Bioengineering and Bioscience 1 article published in JoVE Bioengineering Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics Brandon Alexander Holt1, Quoc D. Mac1, Gabriel A. Kwong1,2,3,4,5 1Wallace H. Coulter Department of Biomedical Engineering, Georgia Tech College of Engineering and Emory School of Medicine, 2Parker H. Petit Institute of Bioengineering and Bioscience, 3Institute for Electronics and Nanotechnology, Georgia Tech, 4Integrated Cancer Research Center, Georgia Tech, 5The Georgia Immunoengineering Consortium, Emory University and Georgia Tech Proteases are tightly regulated enzymes involved in fundamental biological processes, and dysregulated protease activity drives progression of complex diseases such as cancer. This method's goal is to create nanosensors that measure protease activity in vivo by producing a cleavage signal that is detectable from host urine and discriminates disease.
