Atlanta Veterans Affairs Medical Center 3 articles published in JoVE Biology Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope Michael Hamersky IV1, Khushi Tekale1, L. Matthew Winfree2, Matthew J. M. Rowan1,3, Lindsey Seldin1,4,5,6 1Department of Cell Biology, Emory University School of Medicine, 2Independent scholar, 3Center for Neurodegenerative Disease, Emory University School of Medicine, 4Department of Dermatology, Emory University School of Medicine, 5Winship Cancer Institute, Emory University School of Medicine, 6Atlanta Veterans Affairs Medical Center The protocol presents a new tool to simplify intravital imaging using inverted confocal microscopy. Behavior Behavioral Assessment of Visual Function via Optomotor Response and Cognitive Function via Y-Maze in Diabetic Rats Kaavya Gudapati*1,2, Anayesha Singh*1,3, Danielle Clarkson-Townsend1,4, Stephen Q. Phillips1, Amber Douglass1, Andrew J. Feola1,2, Rachael S. Allen1,2 1Center for Visual and Neurocognitive Rehabilitation, Atlanta VA Medical Center, 2Department of Biomedical Engineering, Georgia Institute of Technology, 3Department of Neuroscience, Emory University, 4Gangarosa Department of Environmental Health, Emory University Neural degeneration in both eyes and brain as a result of diabetes can be observed through behavioral tests carried out on rodents. The Y-maze, a measure of spatial cognition, and the optomotor response, a measure of visual function, both provide insight into potential diagnoses and treatments. Bioengineering In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography Rachael S. Allen1,2, Katie Bales1,3, Andrew Feola1,2, Machelle T Pardue1,2,3 1Center of Excellence for Visual and Neurocognitive Rehabilitation, Atlanta Veterans Affairs Medical Center, 2Department of Biomedical Engineering, Georgia Institute of Technology, 3Department of Ophthalmology, Emory University Here, we describe the use of spectral-domain optical coherence tomography (SD-OCT) to visualize retinal and ocular structures in vivo in models of retinal degeneration, glaucoma, diabetic retinopathy, and myopia.
