3 articles published in JoVE
The Hawaii Protocol for Scientific Monitoring of Coffee Berry Borer: a Model for Coffee Agroecosystems Worldwide Melissa Anne Johnson1,2, Robert Hollingsworth2, Samuel Fortna2,3, Luis F. Aristizábal4, Nicholas C. Manoukis2 1Oak Ridge Institute for Science and Education, 2Daniel K. Inouye US Pacific Basin Agricultural Research Center, United States Department of Agriculture-Agricultural Research Service, 3College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, 4Independent Consultant on CBB Management Comprehensive monitoring of coffee berry borer and host plant dynamics is essential for aggregating landscape-level data to improve management of this invasive pest. Here, we present a protocol for scientific monitoring of coffee berry borer movement, infestation, mortality, coffee plant phenology, weather, and farm management via a mobile electronic data recording application.
High Pressure Single Crystal Diffraction at PX^2 Dongzhou Zhang1, Przemyslaw K. Dera1, Peter J. Eng2, Joanne E. Stubbs2, Jin S. Zhang1, Vitali B. Prakapenka2, Mark L. Rivers2 1Hawai’i Institute of Geophysics and Planetology, University of Hawai’i at Manoa, 2Center for Advanced Radiation Sources, University of Chicago In this report, we describe detailed procedures for carrying out single crystal X-ray diffraction experiments with a diamond anvil cell at the GSECARS 13-BM-C beamline at the Advanced Photon Source. ATREX and RSV programs are used to analyze the data.
Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging Shruti Shankar1,2, Meredith E.K. Calvert3,4, Joanne Y. Yew1,2,5 1Temasek Life Sciences Laboratory, 2Department of Biological Science, National University of Singapore, 3Bioimaging and Biocomputing Facility, Temasek Life Sciences Laboratory, 4Histology and Light Microscopy Core, Gladstone Institutes, 5Pacific Biosciences Research Center, University of Hawai'i at Mānoa The forelegs and proboscis of Drosophila contain a rich repertoire of gustatory sensory neurons. Here, we present a method using calcium imaging to measure physiological responses from sensory neurons in the foreleg and proboscis of live flies upon exogenous application of a gustatory pheromone.