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4 articles published in JoVE
Murine Dermal Fibroblast Isolation by FACS Graham G. Walmsley*1,2, Zeshaan N. Maan*1, Michael S. Hu*1,2,3, David A. Atashroo1, Alexander J. Whittam1, Dominik Duscher1, Ruth Tevlin1, Owen Marecic1, H. Peter Lorenz1, Geoffrey C. Gurtner1, Michael T. Longaker1,2 1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 3Department of Surgery, John A. Burns School of Medicine, University of Hawai'i Fibroblast behavior underlies a spectrum of clinical entities, but they remain poorly characterized, largely due to their inherent heterogeneity. Traditional fibroblast research relies upon in vitro manipulation, masking in vivo fibroblast behavior. We describe a FACS-based protocol for the isolation of mouse skin fibroblasts that does not require cell culture.
A Mouse Fetal Skin Model of Scarless Wound Repair Graham G. Walmsley*1,2, Michael S. Hu*1,2,3, Wan Xing Hong1,4, Zeshaan N. Maan1, H. Peter Lorenz1, Michael T. Longaker1,2 1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery, Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, 3Department of Surgery, John A. Burns School of Medicine, University of Hawai'i, 4University of Central Florida College of Medicine During mammalian development, early gestational skin wounds heal without a scar. Here we detail a reliable and reproducible model of fetal scarless wound healing in the cutaneous dorsum of E16.5 (scarless) and E18.5 (scarring) mouse embryos.
Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-κB Reporter Transgenic Whitney Barham1, Taylor Sherrill2, Linda Connelly3, Timothy S. Blackwell2, Fiona E. Yull1 1Cancer Biology Department, Vanderbilt University Medical Center, 2Department of Medicine, Vanderbilt University Medical Center, 3Department of Pharmaceutical Sciences, University of Hawaii at Hilo College of Pharmacy Described here is a technique in which lipopolysaccharide is injected into the lactating mouse mammary gland via the nipple to simulate mastitis, a condition commonly caused by bacterial infection. Lipopolysaccharide injection results in increased nuclear factor kappa B (NF-κB) signaling, visualized through bioluminescent imaging of an NF-κB luciferase reporter mouse.
Introduction to the Ultrasound Targeted Microbubble Destruction Technique Chad B. Walton1, Cynthia D. Anderson1, Rachel Boulay1, Ralph V. Shohet1 1Department of Medicine, JABSOM, University of Hawaii Ultrasound Targeted Microbubble Destruction (UTMD) can be used to direct site-specific delivery of bioactive molecules, including therapeutic genes, to target organs accessible to ultrasound, such as the heart and liver1-6.