2 articles published in JoVE
Anti-RDL and Anti-mGlutR1 Receptors Antibody Testing in Honeybee Brain Sections using CRISPR-Cas9 Irina Sinakevitch1,2, Zev Kurtzman*2, Hyun G. Choi*2, David Arturo Ruiz Pardo3, Romain A. Dahan2, Nathaniel Klein1, Branimir Bugarija4, Erik Wendlandt4, Brian H. Smith2 1Department of Neuroscience, University of Arizona, 2School of Life Sciences, Arizona State University, 3Department of Scientific and Technologic Investigations, University of Sonora, 4Integrated DNA Technologies, Inc. Presented here is a protocol to use the CRISPR-Cas9 system for reducing the production of a protein in the adult honeybee brain to test antibody specificity.
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons Yang Song*1, Xuemei Zhang*1, Lingyan Huang2, Mark A. Behlke2, Andrew Tsourkas1 1Department of Bioengineering, University of Pennsylvania, 2Integrated DNA Technologies, Inc. Ratiometric bimolecular beacons (RBMBs) can be used to image single engineered RNA transcripts in living cells. Here, we describe the preparation and purification of RBMBs, delivery of RBMBs into cells by microporation and fluorescent imaging of single RNA transcripts in real-time.