USDA, Agricultural Research Service 1 article published in JoVE Immunology and Infection Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood Damarius S. Fleming1,2, Sarah J. Anderson1, Laura C. Miller1 1Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, 2ORAU/ORISE Here, we present a protocol optimized for the processing of coding (mRNA) and non-coding (ncRNA) globin reduced RNA-seq libraries from a single whole blood sample.