3 articles published in JoVE
Live-Cell Forward Genetic Approach to Identify and Isolate Developmental Mutants in Chlamydia trachomatis Travis J. Chiarelli1, Nicole A. Grieshaber1, Scott S. Grieshaber1 1Department of Biological Sciences, University of Idaho This protocol utilizes fluorescent promoter-reporters, live-cell microscopy, and individual inclusion extraction in a directed forward genetic approach to identify and isolate developmental mutants of Chlamydia trachomatis.
Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta Joanna Kud1, Wenjie Wang2, Yulin Yuan2, Allan Caplan2, Joseph C. Kuhl2, Louise-Marie Dandurand1, Fangming Xiao2 1Department of Entomology, Plant Pathology and Nematology, University of Idaho, 2Department of Plant Sciences, University of Idaho The goal of this manuscript is to present an outline for the comprehensive biochemical and functional studies of the RING-type E3 ubiquitin ligases. This multistep pipeline, with detailed protocols, validates an enzymatic activity of the tested protein and demonstrates how to link the activity to function.
On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System Joseph B. DeShields1, Rachel A. Bomberger1, James W. Woodhall2, David L. Wheeler1, Natalia Moroz1, Dennis A. Johnson1, Kiwamu Tanaka1 1Department of Plant Pathology, Washington State University, 2Parma Research and Extension Center, University of Idaho Rapid and accurate detection of plant pathogens on-site, especially soil-borne pathogens, is crucial to prevent further inoculum production and proliferation of plant diseases in the field. The method developed here using a portable real-time PCR detection system enables on-site diagnosis under field conditions.