2 articles published in JoVE
Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications Ashim Gupta1, Kevin Sharif2, Megan Walters2, Mia D. Woods1, Anish Potty2, Benjamin J. Main4, Saadiq F. El-Amin III1,2,3 1Department of Medical Microbiology, Immunology & Cell Biology, Southern Illinois University School of Medicine, 2Division of Orthopaedics and Rehabilitation, Department of Surgery, Southern Illinois University School of Medicine, 3Department of Electrical and Computer Engineering, Biomedical Engineering Program, Southern Illinois University Carbondale, 4University of Illinois at Springfield For future applications as a patch to repair partial tears of the Anterior Cruciate Ligament (ACL), human ACL derived cells were isolated from tissue obtained during reconstructive procedures, expanded in vitro and grown on tissue engineered scaffolds. Cellular adhesion and morphology was then performed to confirm biocompatibility on scaffold surface.
In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos Patrick J. Holland1, Angela M. George1, Leslie T.C. Worrell1, Rebecca L. Landsberg1 1Biology Department, University of Illinois at Springfield This study describes the development of an in vitro electroporation technique that allows for the manipulation of gene expression in the lower rhombic lip of midgestation embryos.