Southern Illinois University School of Medicine 5 articles published in JoVE Medicine Trans-Tympanic Drug Delivery for the Treatment of Ototoxicity Kelly Sheehan*1, Sandeep Sheth*2, Debashree Mukherjea1, Leonard P. Rybak1,2, Vickram Ramkumar2 1Department of Surgery, Division of Otolaryngology, Southern Illinois University School of Medicine, 2Department of Pharmacology, Southern Illinois University School of Medicine We present a technique for localized administration of drugs through the trans-tympanic route into the cochlea. Drug delivery through this route would not interfere with the anti-cancer efficacy of the chemotherapeutic drugs such as cisplatin. Cancer Research A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies Katherine A. Murphy*1,2, Britnie R. James*1,2,3, Andrew Wilber4,5, Thomas S. Griffith1,2,3 1Department of Urology, University of Minnesota, 2Masonic Cancer Center, University of Minnesota, 3Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, 4Department of Medical Microbiology, Immunology and Cell Biology, Southern Illinois University School of Medicine, 5Simmons Cancer Institute Implementation of an orthotopic model of renal cell carcinoma in immunocompetent mice affords the investigator a clinically-relevant system defined by the presence of a primary renal tumor and lung metastases in the same animal. This system can be used to preclinically test a variety of treatments in vivo. Neuroscience Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea Scott C. Montgomery1, Brandon C. Cox1,2 1Department of Surgery, Division of Otolaryngology, Southern Illinois University, School of Medicine, 2Department of Pharmacology, Southern Illinois University, School of Medicine We present a method to isolate the adult organ of Corti as three intact cochlear turns (apex, middle, and base). We also demonstrate a procedure for immunostaining with fluorescently tagged antibodies. Together these techniques allow the study of hair cells, supporting cells, and other cell types found in the cochlea. Medicine Tumor Treating Field Therapy in Combination with Bevacizumab for the Treatment of Recurrent Glioblastoma Ayman I. Omar1 1Southern Illinois University School of Medicine A novel methodology that is employed for the treatment of recurrent glioblastomas is described. This treatment approach employs the application of alternating electric tumor treating fields (TTFields), known as TTF Therapy in combination with bevacizumab, a targeted agent that is currently FDA approved as monotherapy. Bioengineering Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications Ashim Gupta1, Kevin Sharif2, Megan Walters2, Mia D. Woods1, Anish Potty2, Benjamin J. Main4, Saadiq F. El-Amin III1,2,3 1Department of Medical Microbiology, Immunology & Cell Biology, Southern Illinois University School of Medicine, 2Division of Orthopaedics and Rehabilitation, Department of Surgery, Southern Illinois University School of Medicine, 3Department of Electrical and Computer Engineering, Biomedical Engineering Program, Southern Illinois University Carbondale, 4University of Illinois at Springfield For future applications as a patch to repair partial tears of the Anterior Cruciate Ligament (ACL), human ACL derived cells were isolated from tissue obtained during reconstructive procedures, expanded in vitro and grown on tissue engineered scaffolds. Cellular adhesion and morphology was then performed to confirm biocompatibility on scaffold surface.
