2 articles published in JoVE
Anaerobic Growth and Maintenance of Mammalian Cell Lines Balbina J. Plotkin1, Ira M. Sigar1, Julie A. Swartzendruber1, Amber Kaminski1 1Department of Microbiology and Immunology, Midwestern University Here, we describe a new method that enables the anaerobic long-term cultivation of established cell lines. The maximum survival time that was tested is 17 days. This method is suitable for the testing of cytotoxic agents and exploring the physiology of anoxically replicating cells.
Adult Mouse DRG Explant and Dissociated Cell Models to Investigate Neuroplasticity and Responses to Environmental Insults Including Viral Infection Michele Fornaro1, Harsh Sharthiya1, Vaibhav Tiwari2 1Department of Anatomy, Chicago College of Osteopathic Medicine (CCOM), Midwestern University, 2Department of Microbiology and Immunology, Chicago College of Osteopathic Medicine (CCOM), Midwestern University In this report, the advantages of organotypic cultures and dissociated primary cultures of mouse-derived dorsal root ganglia are highlighted to investigate a wide range of mechanisms associated with neuron-glial interaction, neuroplasticity, neuroinflammation, and response to viral infection.