2 articles published in JoVE
Magnetic Resonance Imaging Assessment of Carcinogen-induced Murine Bladder Tumors Alexander P. Glaser1, Daniele Procissi2, Yanni Yu3, Joshua J. Meeks3 1Department of Surgery, Division of Urology, NorthShore University HealthSystem, 2Department of Radiology, Northwestern University Feinberg School of Medicine, 3Department of Urology, Northwestern University Feinberg School of Medicine Murine bladder tumors are induced with the N-butyl-N-(4-hydroxybutyl) nitrosamine carcinogen (BBN). Bladder tumor generation is heterogeneous; therefore, an accurate assessment of tumor burden is needed before randomization to experimental treatment. Here we present a fast, reliable MRI protocol to assess tumor size and stage.
Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format Jack K. Phillips1, Sydney A. Sherman1,2, Sevan R. Oungoulian3, John D. Finan1 1Department of Neurosurgery, NorthShore University HealthSystem, 2Midwestern University/Chicago College of Osteopathic Medicine, 3Independent Contractor Here we present a method for a human in vitro model of stretch injury in a 96-well format on a timescale relevant to impact trauma. This includes methods for fabricating stretchable plates, quantifying the mechanical insult, culturing and injuring cells, imaging, and high content analysis to quantify injury.