University of Illinois College of Medicine Rockford

1 article published in JoVE

• Neuroscience

  
  Analyzing Mitochondrial Transport and Morphology in Human Induced Pluripotent Stem Cell-Derived Neurons in Hereditary Spastic Paraplegia Yongchao Mou^1,2, Sukhada Mukte¹, Eric Chai¹, Joshua Dein³, Xue-Jun Li^{1,2 1}Department of Biomedical Sciences, University of Illinois College of Medicine Rockford, ²Department of Bioengineering, University of Illinois at Chicago, ³MD Program, University of Illinois College of Medicine Rockford Impaired mitochondrial transport and morphology are involved in various neurodegenerative diseases. The presented protocol uses induced pluripotent stem cell-derived forebrain neurons to assess mitochondrial transport and morphology in hereditary spastic paraplegia. This protocol allows characterization of mitochondrial trafficking along axons and analysis of their morphology, which will facilitate the study of neurodegenerative disease.