1 article published in JoVE

• Biology

  
  A Proximal Culture Method to Study Paracrine Signaling Between Cells Subramanyam Dasari¹, Taruni Pandhiri¹, James Haley¹, Dean Lenz², Anirban K. Mitra^{1,3,4 1}Indiana University School of Medicine, ²Urology, Indiana University Health Southern Indiana Physicians, ³Indiana University Melvin and Bren Simon Cancer Center, ⁴Department of Medical and Molecular Genetics, Indiana University School of Medicine Paracrine and juxtacrine cellular interactions play an important role in many biological processes, including tumor progression, immune responses, angiogenesis, and development. Here, a proximal culture method is used to study paracrine signaling where the localized concentrations of the secreted factors are maintained while preventing direct cellular contact.