Eli Lilly and Company

2 articles published in JoVE

• Biology

  
  Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae Bryce W. Buchanan¹, Michael E. Lloyd^1,2, Sarah M. Engle¹, Eric M. Rubenstein^{1 1}Department of Biology, Ball State University, ²Bioproduct Research & Development, Eli Lilly and Company Protein abundance reflects the rates of both protein synthesis and protein degradation. This article describes the use of cycloheximide chase followed by western blotting to analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast).

• Bioengineering

  
  Drug-induced Sensitization of Adenylyl Cyclase: Assay Streamlining and Miniaturization for Small Molecule and siRNA Screening Applications Jason M. Conley¹, Tarsis F. Brust¹, Ruqiang Xu¹, Kevin D. Burris², Val J. Watts^{1 1}Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, ²Quantitative Biology, Eli Lilly and Company Persistent activation of inhibitory G protein-coupled receptors results in sensitization of adenylyl cyclase signaling. To identify the essential molecular pathways, nonbiased approaches are necessary; however, this strategy requires the development of a scalable cell-based cAMP sensitization assay. Herein, we describe a sensitization assay for small molecule and siRNA screening.