Indiana University Health 1 article published in JoVE Biology qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue Morgan H. McCoy1, Kristin Post2, Joyashree D. Sen2, Hsim Y. Chang2, Zijin Zhao1, Rong Fan1, Shaoxiong Chen1, Diane Leland1, Liang Cheng1, Jingmei Lin1 1Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, 2Department of Pathology and Laboratory Medicine, Indiana University Health This protocol describes qPCR detection of cytomegalovirus in formalin-fixed, paraffin-embedded biopsy tissue, which is rapid, sensitive, specific, and useful for interpreting equivocal hematoxylin and eosin or immunohistochemical staining patterns.
