2 articles published in JoVE
A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering Christopher A. Neal1, Jennifer G. Nelson-Brantley1, Michael S. Detamore2, Hinrich Staecker1, Adam J. Mellott3 1Department of Otolaryngology, University of Kansas Medical Center, 2Stephenson School of Biomedical Engineering, University of Oklahoma, 3Department of Plastic Surgery, University of Kansas Medical Center The goal of this protocol is to demonstrate an effective method to decellularize and decalcify mouse cochleae for utilization as scaffolds for tissue engineering applications.
Methods and Tips for Intravenous Administration of Adeno-associated Virus to Rats and Evaluation of Central Nervous System Transduction Mychal S. Grames1, Kasey L. Jackson1, Robert D. Dayton1, John A. Stanford2, Ronald L. Klein1 1Department of Pharmacology, Toxicology, and Neuroscience, Louisiana State University Health Sciences Center, 2Department of Molecular & Integrative Physiology, University of Kansas Medical Center Methods for a wide-scale central nervous system gene delivery in the rat are covered. In this example, the purpose is to mimic a disease that affects the entire spinal cord. The widespread transduction can be used to deliver a therapeutic protein to the CNS from a one-time, peripheral administration.