2 articles published in JoVE
Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory Thea M. Edwards1,2, Howard E. Morgan2,3, Coralia Balasca4, Naveen K. Chalasani5, Lauren Yam4,6, Alison M. Roark4 1Department of Biology, University of the South, 2School of Biological Sciences, Louisiana Tech University, 3School of Medicine, Louisiana State University Health Sciences Center, 4Department of Biology, Furman University, 5Department of Computer Science, Louisiana Tech University, 6Clemson University This article presents an optimized yeast estrogen screen for quantifying ligands in Personal Care Products (PCPs) that bind estrogen receptors alpha (ERα) and/or beta (ERβ). The method incorporates two colorimetric substrate options, a six-day refrigerated incubation for use in undergraduate courses, and statistical tools for data analysis.
Multiphoton Microscopy of Cleared Mouse Brain Expressing YFP Sonia G. Parra*1, Sam S. Vesuna*1, Teresa A. Murray1,2, Michael J. Levene1 1Department of Biomedical Engineering, Yale University, 2Department of Biomedical Engineering, Louisiana Tech University Multiphoton microscopy of whole mouse organs is possible by optically clearing the organ before imaging, but not all protocols preserve the fluorescent signal of fluorescent proteins. Using an optical clearing method with ethanol-based dehydration and benzyl alcohol:benzyl benzoate clearing, we show high-resolution multiphoton images of whole mouse brain expressing YFP.