2 articles published in JoVE
A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas Teresa A. Hudock1, Deepak Kaushal1,2 1Bacteriology and Parasitology, Tulane National Primate Research Center, 2Microbiology and Immunology, Tulane National Primate Research Center Microdissection has been extensively employed for the examination of DNA, RNA, and protein within tissue. Laser capture microscopy (LCM) is the most commonly used method, but a new milling technique, mesodissection, is recently available. We demonstrate RNA extraction from mesodissected formalin fixed paraffin embedded tissue slides of Mycobacterium tuberculosis granulomas.
Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor Ryan W. Bonvillain1,2, Michelle E. Scarritt1, Nicholas C. Pashos1, Jacques P. Mayeux1, Christopher L. Meshberger1, Aline M. Betancourt1,3, Deborah E. Sullivan1,3, Bruce A. Bunnell1,2,4 1Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 2Division of Regenerative Medicine, Tulane National Primate Research Center, 3Department of Microbiology and Immunology, Tulane University School of Medicine, 4Department of Pharmacology, Tulane University School of Medicine Whole-organ decellularization produces natural biological scaffolds that may be used for regenerative medicine. The description of a nonhuman primate model of lung regeneration in which whole lungs are decellularized and then seeded with adult stem cells and endothelial cells in a bioreactor that facilitates vascular circulation and liquid media ventilation is presented.