3 articles published in JoVE
Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System Loren M. Brown1, Katherine L. Hebert2, Rakesh R. Gurrala3, C. Ethan Byrne4, Matthew Burow5, Elizabeth C. Martin4, Frank H. Lau1 1Department of Surgery, Louisiana State University Health Sciences Center, 2Department of Bioinnovation, Tulane University, 3Tulane University School of Medicine, 4Department of Biological and Agricultural Engineering, Louisiana State University, 5Department of Medicine, Tulane University School of Medicine This protocol describes the construction of an in vitro microphysiological system for studying breast cancer using primary human breast tissue with off the shelf materials.
Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector Shangyu Gong*1, Yichi Zhang*1, Hongcun Bao1, Xianfeng Wang1, Chih-Hsuan Chang1, Yi-Chun Huang1, Wu-Min Deng1 1Department of Biochemistry and Molecular Biology, Tulane University School of Medicine This protocol provides detailed guidance for the initial and continued generational allotransplantation of Drosophila tumors into the abdomen of adult hosts for studying various aspects of neoplasia. Using an autoinjector apparatus, researchers can achieve improved efficiency and tumor yields compared to those achieved by traditional, manual methods.
A Contemporary Warming/Restraining Device for Efficient Tail Vein Injections in a Murine Fungal Sepsis Model Junko Yano1, Elizabeth A. Lilly2, Mairi C. Noverr2, Paul L. Fidel1 1Center for Oral and Craniofacial Biology, Louisiana State University Health-School of Dentistry, 2Department of Microbiology and Immunology, Tulane University School of Medicine Here, we present an effective and efficient method for rodent tail vein injections using a uniquely designed warming/restraining device. By streamlining the initiation of vasodilation and restraining processes, this protocol allows accurate and timely intravenous injections of large groups of animals with minimal distress.