2 articles published in JoVE
Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae Felix Ellett1, Daniel Irimia1 1BioMEMS Resource Center, Department of Surgery, Massachusetts General Hospital–Harvard Medical School–Shriners Burns Hospital Microinjection of zebrafish embryos and larvae is a crucial but challenging technique used in many zebrafish models. Here, we present a range of microscale tools to aid in the stabilization and orientation of zebrafish for both microinjection and imaging.
Ex Situ Normothermic Machine Perfusion of Donor Livers Negin Karimian1,2, Alix P.M. Matton1,2, Andrie C. Westerkamp1,2, Laura C. Burlage1,2, Sanna op den Dries1,2, Henri G.D. Leuvenink2, Ton Lisman1,2, Korkut Uygun3, James F. Markmann4, Robert J. Porte1,2 1Section of Hepato-Pancreato-Biliary Surgery and Liver Transplantation, University of Groningen, University Medical Center Groningen, 2Surgical Research Laboratory, Department of Surgery, University of Groningen, University Medical Center Groningen, 3Center of Engineering in Medicine/Surgical Services, Massachusetts General Hospital, Harvard Medical School, and Shriners Burns Hospital, 4Division of Transplantation, Department of Surgery, Massachusetts General Hospital, Harvard Medical School Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.