3 articles published in JoVE
A Pipeline using Bilateral In Utero Electroporation to Interrogate Genetic Influences on Rodent Behavior Ashley L. Comer1,2,3,4, Balaji Sriram5, William W. Yen2, Alberto Cruz-Martín1,2,3,4,6 1The Graduate Program for Neuroscience, Boston University, 2Department of Biology, Boston University, 3Neurophotonics Center, Boston University, 4Center for Systems Neuroscience, Boston University, 5Research and Early Development, Biogen, 6Department Pharmacology and Experimental Therapeutics, Boston University The role of recently discovered disease-associated genes in the pathogenesis of neuropsychiatric disorders remains obscure. A modified bilateral in utero electroporation technique allows for the gene transfer in large populations of neurons and examination of the causative effects of gene expression changes on social behavior.
A Microbiomechanical System for Studying Varicosity Formation and Recovery in Central Neuron Axons Dustin Servello1, Yuanzheng Gu2,3, Chen Gu1,2 1Molecular, Cellular, and Developmental Biology Program, The Ohio State University, 2Department of Biological Chemistry and Pharmacology, The Ohio State University, 3Biogen This protocol describes a physiologically relevant, pressurized fluid approach for rapid and reversible induction of varicosities in neurons.
RNAi Trigger Delivery into Anopheles gambiae Pupae Kimberly Regna1, Rachel M. Harrison1, Shannon A. Heyse1, Thomas C. Chiles1, Kristin Michel2, Marc A. T. Muskavitch1,3 1Biology Department, Boston College, 2Division of Biology, Kansas State University, 3Discovery Research, Biogen RNA interference (RNAi) is an extremely valuable tool for uncovering gene function. However, the ability to target genes using RNAi during pre-adult stages is limited in the major human malaria vector Anopheles gambiae. We describe an RNAi protocol to reduce gene function via direct injection during pupal development.