2 articles published in JoVE
The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan Adam B. Cornwell1, Jesse R. Llop1, Peter Salzman2,3, Juilee Thakar4, Andrew V. Samuelson1 1Department of Biomedical Genetics, University of Rochester Medical Center, 2Department of Biostatistics and Computational Biology, University of Rochester Medical Center, 3Non-Clinical Statistics, Bristol-Myers Squibb, 4Department of Microbiology and Immunology, University of Rochester Medical Center Here we describe the Replica Set method, an approach to quantitatively measure C. elegans lifespan/survival and healthspan in a high-throughput and robust manner, thus allowing screening of many conditions without sacrificing data quality. This protocol details the strategy and provides a software tool for analysis of Replica Set data.
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood Yoshinobu Koguchi1, Iliana L. Gonzalez1, Tanisha L. Meeuwsen1, William L. Miller1, Daniel P. Haley1,2, Alice N. Tanibata-Branham3, Keith S. Bahjat1,4 1Human Immune Monitoring Laboratory, Earle A. Chiles Research Institute, Providence Cancer Center, Providence Portland Medical Center, 2Sony Biotechnology, 3Beckman Coulter, Inc. Life Sciences, 4Bristol-Myers Squibb Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.