Wyss Institute for Biologically Inspired Engineering at Harvard University 2 articles published in JoVE Bioengineering Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens Daniel J. Wiegand1,2, Henry H. Lee1, Nili Ostrov*1, George M. Church*1,2 1Department of Genetics, Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering Cell-free expression systems are powerful and cost-efficient tools for the high-throughput synthesis and screening of important proteins. Here, we describe the preparation of cell-free protein expression system using Vibrio natriegens for the rapid protein production using plasmid DNA, linear DNA, and mRNA template. Bioengineering Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device Hyun Jung Kim1, Jaewon Lee1, Jin-Ha Choi1, Anthony Bahinski2, Donald E. Ingber2,3,4 1Department of Biomedical Engineering, The University of Texas at Austin, 2Wyss Institute for Biologically Inspired Engineering at Harvard University, 3 We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.
