Johns Hopkins Hospital 4 articles published in JoVE Neuroscience Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq Kurt Weir*1, Patrick Leavey*1, Clayton Santiago1, Seth Blackshaw1,2,3,4,5,6 1Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Psychiatry and Behavioral Science, The Johns Hopkins Hospital, 3Department of Ophthalmology, The Johns Hopkins Hospital, 4Department of Neurology, The Johns Hopkins Hospital, 5Institute for Cell Engineering, Johns Hopkins University School of Medicine, 6Kavli Neuroscience Discovery Institute, Johns Hopkins University School of Medicine Here, the authors showcase the utility of MULTI-seq for phenotyping and subsequent paired scRNA-seq and scATAC-seq in characterizing the transcriptomic and chromatin accessibility profiles in retina. Bioengineering A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation Yang Bai1,2, Enoch Yeung2, Cecillia Lui2, Chin Siang Ong2, Isaree Pitaktong2, Chenyu Huang2, Takahiro Inoue2, Hiroshi Matsushita2, Chunye Ma2, Narutoshi Hibino2 1Department of Cardiac Surgery, The First Hospital of Jilin University, 2Division of Cardiac Surgery, Johns Hopkins Hospital This protocol describes a net mold-based method to create three-dimensional scaffold-free cardiac tissues with satisfactory structural integrity and synchronous beating behavior. Bioengineering Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting Chin Siang Ong1,2, Takuma Fukunishi1, Andrew Nashed1, Adriana Blazeski3, Huaitao Zhang1, Samantha Hardy1, Deborah DiSilvestre2, Luca Vricella1, John Conte1, Leslie Tung3, Gordon Tomaselli2, Narutoshi Hibino1 1Division of Cardiac Surgery, Johns Hopkins Hospital, 2Division of Cardiology, Johns Hopkins Hospital, 3Department of Biomedical Engineering, Johns Hopkins University This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease. Medicine Dual-phase Cone-beam Computed Tomography to See, Reach, and Treat Hepatocellular Carcinoma during Drug-eluting Beads Transarterial Chemo-embolization Vania Tacher1, MingDe Lin2, Nikhil Bhagat1, Nadine Abi Jaoudeh3, Alessandro Radaelli4, Niels Noordhoek4, Bart Carelsen4, Bradford J. Wood3, Jean-François Geschwind1 1Russell H. Morgan Department of Radiology and Radiological Science, Division of Vascular and Interventional Radiology, The Johns Hopkins Hospital, 2Clinical Informatics, Interventional, and Translational Solutions (CIITS), Philips Research North America, 3Center for Interventional Oncology, Interventional Radiology Section, National Institutes of Health, 4Interventional X-ray, Philips Healthcare Dual-phase cone-beam computed tomography (DP-CBCT) is a useful intraprocedural imaging technique for transarterial chemo-embolization treatment with drug-eluting beads of hepatocellular carcinoma. DP-CBCT has been used to perform three major steps in oncologic interventional radiology: tumor localization (see), navigation and intraprocedural catheter guidance (reach), and intraprocedural evaluation of treatment success (treat).
