1 article published in JoVE

• Bioengineering

  
  Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System Anjali Nandal^1,2, Barbara Mallon³, Bhanu P. Telugu^{1,2,4 1}Department of Animal and Avian Sciences, University of Maryland, ²Animal Bioscience and Biotechnology Laboratory, ARS, USDA, ³NIH Stem Cell Unit, Bethesda, National Institutes of Health, ⁴RenOVAte Biosciences Inc This protocol describes in detail the generation of footprint-free induced pluripotent stem cells (iPSCs) from human pancreatic cells in feeder-free conditions, followed by editing using CRISPR/Cas9 ribonucleoproteins and characterization of the modified single-cell clones.